Top trophic level predators are at risk from bioaccumulation of heavy metals from their prey. Using nondestructively collected tissues as a method of assessing metal concentrations in snakes is useful for populations that are threatened or declining. This paper reports concentrations of arsenic (As), cadmium (Cd), chromium (Cr), lead (Pb), mercury (Hg), and selenium (Se) in tissues of Northern pine snakes (Pituophis melanoleucus) from the New Jersey Pine Barrens, a relatively pristine, undisturbed habitat. We also determined if skin is an appropriate indicator of internal concentrations and identified the factors (tissue, year of collection, length, sex) that might explain variations in metal concentrations. Because they can grow to 2-m long and live for 25 years, we suggest that these snakes might accumulate heavy metals. Multiple regression models were significant, explaining 16% (lead) to 61% (mercury) of variation by tissue type. For mercury and chromium, size also was significant. The highest concentrations were in liver and kidney for all metals, except chromium and lead. Mercury concentrations in tissues were within the range reported for other snakes and were below effects concentrations in reptiles. The concentrations in skin were correlated with all internal tissues for mercury and for all internal tissues except heart for cadmium. These data show that shed skin can be used as an indicator of metals in pine snakes and that, at present, concentrations of heavy metals in this population are within the range of those found in other snake species from uncontaminated sites.