Human Leukocyte Antigen (HLA) Class I and II Polymorphism in Iranian Healthy Population from Yazd Province

Behrouz Nikbin; Mohammad Hossein Nicknam; Hossein Hadinedoushan; Bita Ansaripour; Batol Moradi; Mirsaeed Yekaninejad; Mahdi Aminikhah; Mohammad Mehdi Ranjbar; Aliakbar Amirzargar

Human Leukocyte Antigen (HLA) Class I and II Polymorphism in Iranian Healthy Population from Yazd Province

Iran J Allergy Asthma Immunol. 2017 Feb;16(1):1-13.

Authors

Behrouz Nikbin¹, Mohammad Hossein Nicknam¹, Hossein Hadinedoushan², Bita Ansaripour³, Batol Moradi³, Mirsaeed Yekaninejad⁴, Mahdi Aminikhah⁴, Mohammad Mehdi Ranjbar⁵, Aliakbar Amirzargar¹

Affiliations

¹ Molecular Immunology Research Center, Tehran University of Medical Sciences, Tehran, Iran AND Immunogenetic Laboratory, Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
² Department of Immunology, Shahid Sadoughi University of Medical Sciences and Health Services, Yazd, Iran.
³ Immunogenetic Laboratory, Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
⁴ Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
⁵ Department of Immunology, Razi Vaccine and Serum Research Institute, Karaj, Iran.

PMID: 28417619

Abstract

The major histocompatibility complex (MHC) genes are the most polymorphic loci in the human genome and have been widely studied in various populations and ethnic groups. Investigations into the HLA genes and proteins have been useful tool for anthropological, transplantation and disease association studies. The polymorphism of the HLA class I (A, B, C) and class II (DRB1, DQA1, DQB1) genes were investigated in 90 unrelated Iranian individuals from Yazd province located in the center of Iran using sequence-specific primers (PCR-SSP). Allele and haplotype frequencies, expected/observed heterozygosity, unbiased expected heterozygosity, number of effective alleles, deviations from Hardy-Weinberg (HW) equilibrium and genetic diversity were computed. A total of 23, 48, 23, 24, 13 and 16 alleles for HLA-A, -B,-C, -DRB1, -DQA and -DQB loci were determined, respectively in the population study. The most frequent allele identified in this study were A*02:01 (18.889%), HLA-B* 51:01 (12.778%), HLA-C* 12:03 (17.033%), HLA-DRB* 11 (24.4%), HLA-DQA* 05:05 (20.55%) and HLA-DQB*03:01 (22.8%).Furthermore, the most frequent 3-locus haplotypes were DRB*11-DQA*05:01-DQB*03:01 (21.1%), HLA-A*02:01- B *50:01- DRB*07:01 (4.9%) and A*26:01 -B* 38:01 -C*12:03(5%). The most 4-locus haplotype were A*11:01 -B* 52:01 -C*12:03 -DRB!*15(2.5%) and A*02:01 -B* 50:01 -DRB1*07:01 -DQB1*02:01(4.5%). The heterozygosity of the study population was confirmed the expected value and not deviated from Hardy-Weinberg equilibrium for all loci (p>0.05). Our study shows a close relatedness between Yazd population and other ethnic group of Iran despite some differences, which may be due to admixture of each one of these groups with each other or foreigner subpopulations during centuries. Moreover, the results of this study suggest that the Iranian population from Yazd province is in close vicinity with the Caucasians populations and far from the Korean and Japanese populations.

Keywords: Class I and II; Keywords: Human leukocyte antigen (HLA); Polymorphism; Yazd province.

MeSH terms

Alleles
Female
Gene Frequency
Genetics, Population*
Genotyping Techniques
Geography
Haplotypes
Healthy Volunteers
Histocompatibility Antigens Class I / genetics*
Histocompatibility Antigens Class II / genetics*
Humans
Iran / epidemiology
Male
Polymorphism, Genetic*
Population Surveillance

Substances

Histocompatibility Antigens Class I
Histocompatibility Antigens Class II