A wireless point-of-care testing system for the detection of neuron-specific enolase with microfluidic paper-based analytical devices

Yan Fan; Juntao Liu; Yang Wang; Jinping Luo; Huiren Xu; Shengwei Xu; Xinxia Cai

doi:10.1016/j.bios.2017.04.003

A wireless point-of-care testing system for the detection of neuron-specific enolase with microfluidic paper-based analytical devices

Biosens Bioelectron. 2017 Sep 15:95:60-66. doi: 10.1016/j.bios.2017.04.003. Epub 2017 Apr 12.

Authors

Yan Fan¹, Juntao Liu², Yang Wang², Jinping Luo², Huiren Xu², Shengwei Xu², Xinxia Cai³

Affiliations

¹ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China; Intelligence and Information Engineering College of Tangshan University, Tangshan 063000, China.
² State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China.
³ State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China; University of Chinese Academy of Sciences, Beijing 10090, China. Electronic address: xxcai@mail.ie.ac.cn.

PMID: 28412662
DOI: 10.1016/j.bios.2017.04.003

Abstract

Neuron-specific enolase (NSE) had clinical significance on diagnosis, staging, monitoring effect and judging prognosis of small cell lung cancer. Thus, there had a growing demand for the on-site testing of NSE. Here, a wireless point-of-care testing (POCT) system with electrochemical measurement for NSE detection was developed and verified. The wireless POCT system consisted of microfluidic paper-based analytical devices (μPADs), electrochemical detector and Android's smartphone. Differential pulse voltammetry (DPV) measurement was adopted by means of electrochemical detector which including a potentiostat and current-to-voltage converter. μPADs were modified with nanocomposites synthesized by Amino functional graphene, thionine and gold nanoparticles (NH₂-G/Thi/AuNPs) as immunosensors for NSE detection. Combined with μPADs, the performance of the wireless POCT system was evaluated. The peak currents showed good linear relationship of the logarithm of NSE concentration ranging from 1 to 500ngmL^-1 with the limit of detection (LOD) of 10pgmL^-1. The detection results were automatically stored in EEPROM memory and could be displayed on Android's smartphone through Bluetooth in real time. The detection results were comparable to those measured by a commercial electrochemical workstation. The wireless POCT system had the potential for on-site testing of other tumor markers.

Keywords: Electrochemical; Neuron-specific enolase; Point-of-care testing; Smartphone; Wireless.

MeSH terms

Biosensing Techniques*
Graphite / chemistry
Humans
Limit of Detection
Metal Nanoparticles / chemistry*
Microfluidic Analytical Techniques*
Paper
Phosphopyruvate Hydratase / chemistry
Phosphopyruvate Hydratase / isolation & purification*
Point-of-Care Systems

Substances

Graphite
Phosphopyruvate Hydratase