Knockdown of Nucleostemin in an ovarian cancer SKOV-3 cell line and its effects on cell malignancy

Biochem Biophys Res Commun. 2017 May 27;487(2):262-267. doi: 10.1016/j.bbrc.2017.04.046. Epub 2017 Apr 12.

Abstract

Nucleostemin plays an essential role in the proliferation of some stem cells and cancer cells, but no research has been conducted to assess the link between Nucleostemin and ovarian cancer. To investigate the role of Nucleostemin in ovarian cancer tumorigenesis, we generated short hairpin (sh)RNA to knockdown the expression of the Nucleostemin gene in an ovarian cancer SKOV-3 cell line. We found that knockdown of this gene led to cell-cycle arrest, as well as to an increase in apoptosis. In addition, migration and invasion demonstrated significant inhibition in Nucleostemin-deficient cells. Furthermore, the knockdown of Nucleostemin dramatically suppressed xenograft progression in BALB/c nude mice. Our findings suggest that Nucleostemin is associated with malignancy in an ovarian cancer SKOV-3 cell line.

Keywords: Malignancy; Nucleostemin; Ovarian cancer.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Cycle Checkpoints*
  • Cell Line, Tumor
  • Cell Proliferation*
  • Disease Progression
  • Female
  • GTP-Binding Proteins / genetics
  • GTP-Binding Proteins / metabolism*
  • Gene Knockdown Techniques
  • Humans
  • Mice, Inbred BALB C
  • Mice, Nude
  • Neoplasm Invasiveness
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism*
  • Ovarian Neoplasms / metabolism*
  • Ovarian Neoplasms / pathology
  • Ovarian Neoplasms / secondary*

Substances

  • GNL3 protein, human
  • Nuclear Proteins
  • GTP-Binding Proteins