Purification, in vitro antioxidant and in vivo anti-aging activities of exopolysaccharides by Agrocybe cylindracea

Int J Biol Macromol. 2017 Sep:102:351-357. doi: 10.1016/j.ijbiomac.2017.04.039. Epub 2017 Apr 12.

Abstract

The in vitro antioxidant and in vivo anti-aging activities in D-galactose-induced aging mice of exopolysaccharides (EPS) and its fractions (EPS-1, EPS-2 and EPS-3) by Agrocybe cylindraceas were investigated presently. The scavenging effects of EPS-2 on hydroxyl (HO) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 97.1±4.25% and 90.23±3.25%, while the reducing power reached 1.18±0.08 at 120mg/L, which were higher than EPS, EPS-1 and EPS-3 at 1000mg/L. The EPS showed potential anti-aging activities via reducing the contents of malonaldehyde (MDA) and total cholesterol (TC), as well as increasing the activities of superoxide dismutase (SOD) activities, glutathione peroxidase (GSH-Px) activities, and total antioxidant capacity (T-AOC). Additionally, the monosaccharide analysis showed EPS contained rhamnose and glucose with the molar ratio of 2.8:1. These results demonstrated that EPS could be used as functional foods and natural drugs on preventing and mitigating the aging process.

Keywords: Agrocybe cylindraceas; Anti-aging activities; Antioxidant activities; Exopolysaccharides.

MeSH terms

  • Aging / drug effects*
  • Agrocybe / chemistry*
  • Animals
  • Antioxidants / chemistry
  • Antioxidants / isolation & purification*
  • Antioxidants / pharmacology*
  • Biphenyl Compounds / chemistry
  • Fungal Polysaccharides / chemistry
  • Fungal Polysaccharides / isolation & purification*
  • Fungal Polysaccharides / pharmacology*
  • Hydroxyl Radical / chemistry
  • Male
  • Mice
  • Monosaccharides / analysis
  • Picrates / chemistry

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Fungal Polysaccharides
  • Monosaccharides
  • Picrates
  • Hydroxyl Radical
  • 1,1-diphenyl-2-picrylhydrazyl