Yacon (Smallanthus sonchifolius) and Lactobacillus acidophilus CRL 1014 reduce the early phases of colon carcinogenesis in male Wistar rats

Ana Paula da Silva Almeida; Camilla Martins Avi; Luís Fernando Barbisan; Nelci Antunes de Moura; Brunno Felipe Ramos Caetano; Guilherme Ribeiro Romualdo; Kátia Sivieri

doi:10.1016/j.foodres.2015.04.034

Yacon (Smallanthus sonchifolius) and Lactobacillus acidophilus CRL 1014 reduce the early phases of colon carcinogenesis in male Wistar rats

Food Res Int. 2015 Aug:74:48-54. doi: 10.1016/j.foodres.2015.04.034. Epub 2015 Apr 24.

Authors

Ana Paula da Silva Almeida¹, Camilla Martins Avi¹, Luís Fernando Barbisan², Nelci Antunes de Moura², Brunno Felipe Ramos Caetano², Guilherme Ribeiro Romualdo², Kátia Sivieri³

Affiliations

¹ Department of Food and Nutrition, Faculty of Pharmaceutical Science, UNESP - UnivEstadual Paulista Araraquara, SP, Brazil.
² Department of Morphology, Institute of Biosciences, UNESP-São Paulo State University, Botucatu, SP, Brazil.
³ Department of Food and Nutrition, Faculty of Pharmaceutical Science, UNESP - UnivEstadual Paulista Araraquara, SP, Brazil. Electronic address: katiasiv@hotmail.com.

PMID: 28412002
DOI: 10.1016/j.foodres.2015.04.034

Abstract

The modifying effects of aqueous yacon extract (AYE) and Lactobacillus acidophilus CRL 1014 against colon carcinogenesis induced by 1,2-dimethylhydrazine (DMH) in male Wistar rats were investigated. Animals were allocated into five groups: G1: untreated group; G2: DMH-treated group; G3: DMH+L. acidophilus-treated group; G4: DMH+AYE-treated group; G5: DMH+L. acidophilus and AYE-treated group. A significant reduction (p<0.05) in leukocyte DNA damage and in colonic cell proliferation was observed after the first DMH administration in G3 (probiotic), G4 (prebiotic) and G5 (synbiotic) groups. In this moment, a significant increase (p<0.05) in colonic apoptosis was also observed in G3 (probiotic) and G5 (synbiotic) groups. In special, at five months after DMH administrations, a significant reduction (p<0.05) in ACF development was observed in G3 (probiotic), G4 (prebiotic) and G5 (synbiotic) groups. Incidence of colon tumors was lower at five months in G4 (prebiotic) and G5 (synbiotic) groups but not in eight months after DMH administrations. In conclusion, the findings suggest that the oral treatments have potential effects as a chemopreventive agent against colon carcinogenesis on an early stage of tumor development.

Keywords: Chemical carcinogenesis; Colon; Prebiotic; Probiotic; Synbiotic.