In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits

Yong-Seo Park; Milena Cvikrová; Olga Martincová; Kyung-Sik Ham; Seong-Gook Kang; Yang-Kyun Park; Jacek Namiesnik; Adamo Domenico Rombolà; Zenon Jastrzebski; Shela Gorinstein

doi:10.1016/j.foodres.2015.04.021

In vitro antioxidative and binding properties of phenolics in traditional, citrus and exotic fruits

Food Res Int. 2015 Aug:74:37-47. doi: 10.1016/j.foodres.2015.04.021. Epub 2015 Apr 20.

Affiliations

¹ Department of Horticultural Science, Mokpo National University, Muan, Jeonnam, Republic of Korea. Electronic address: ypark@mokpo.ac.kr.
² Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic.
³ Department of Food Engineering, Mokpo National University, Muan, Jeonnam, Republic of Korea.
⁴ Department of Analytical Chemistry, Chemical Faculty, Gdańsk University of Technology, 80 952 Gdańsk, Poland.
⁵ Department of Agricultural Sciences, University of Bologna, 40127 Bologna, Italy.
⁶ Department of Pharmacology, National Medicines Institute, 00-725 Warsaw, Poland.
⁷ The Institute for Drug Research, School of Pharmacy, The Hebrew University, Hadassah Medical School, Jerusalem 91120, Israel. Electronic address: shela.gorin@mail.huji.ac.il.

PMID: 28412001
DOI: 10.1016/j.foodres.2015.04.021

Abstract

Many polyphenols bind proteins, therefore our research was focused on the potential of protein binding to polyphenols of investigated fruits and their health-related effects. The contents of polyphenols and related antioxidant activities of traditional, citrus and exotic fruits were compared. The presence of polyphenols (flavonoids and phenolic acids) in the investigated samples and their interaction with human serum albumin (HSA) was studied by HPLC, Fourier Transform Infrared (FT-IR) and three dimensional fluorescence spectroscopy (3D-FL). The highest levels of polyphenols, antioxidant and binding capacities were found in red and blond grapefruits (citrus group), followed by strawberries and apples (traditional group) and mangosteen and kiwi fruit (exotic fruit), which also contained the highest levels of protocatechuic, p-coumaric, ferulic acids and quercetin. In conclusion, for the first time, the interaction of the polyphenols with human serum albumin was evaluated by fluorometry/FTIR. The obtained binding profiles allowed the comparison of three different groups of fruits. A mixture of these fruits can be recommended for consumption.

Keywords: Antioxidant; Binding properties; Bioactivity; Fluorescence; Fruits.