Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

Yu Chen; Zhuqing Peng; Chao Wu; Zhihui Ma; Guochang Ding; Guangqiu Cao; Shaoning Ruan; Sizu Lin

doi:10.1371/journal.pone.0175571

Genetic diversity and variation of Chinese fir from Fujian province and Taiwan, China, based on ISSR markers

PLoS One. 2017 Apr 13;12(4):e0175571. doi: 10.1371/journal.pone.0175571. eCollection 2017.

Authors

Yu Chen^{1

2}, Zhuqing Peng³, Chao Wu^{2

4}, Zhihui Ma⁵, Guochang Ding², Guangqiu Cao^{1

2}, Shaoning Ruan^{1

2}, Sizu Lin^{1

2}

Affiliations

¹ College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
² State Forestry Administration Engineering Research Center of Chinese Fir, Fuzhou, Fujian, China.
³ Department of Nature, Fujian Museum, Fuzhou, Fujian, China.
⁴ College of Computer and Information Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
⁵ College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.

Abstract

Genetic diversity and variation among 11 populations of Chinese fir from Fujian province and Taiwan were assessed using inter-simple sequence repeat (ISSR) markers to reveal the evolutionary relationship in their distribution range in this report. Analysis of genetic parameters of the different populations showed that populations in Fujian province exhibited a greater level of genetic diversity than did the populations in Taiwan. Compared to Taiwan populations, significant limited gene flow were observed among Fujian populations. An UPGMA cluster analysis showed that the most individuals of Taiwan populations formed a single cluster, whereas 6 discrete clusters were formed by each population from Fujian. All populations were divided into 3 main groups and that all 5 populations from Taiwan were gathered into a subgroup combined with 2 populations, Dehua and Liancheng, formed one of the 3 main groups, which indicated relative stronger relatedness. It is supported by a genetic structure analysis. All those results are suggesting different levels of genetic diversity and variation of Chinese fir between Fujian and Taiwan, and indicating different patterns of evolutionary process and local environmental adaption.

MeSH terms

China
Cluster Analysis
Cunninghamia / genetics*
DNA, Plant / genetics*
Evolution, Molecular
Gene Flow
Genetic Variation
Microsatellite Repeats*
Taiwan

Substances

DNA, Plant

Grants and funding

Funding for this report was provided by central financial support for local special program “Chinese fir plantation in colleges and universities cultivate innovative team building” (6213c0111). The corresponding author Sizu Lin is the recipient.