Nitrogen removal performance and loading capacity of a novel single-stage nitritation-anammox system with syntrophic micro-granules

Shaopo Wang; Yuan Liu; Qigui Niu; Jiayuan Ji; Toshimasa Hojo; Yu-You Li

doi:10.1016/j.biortech.2017.03.164

Nitrogen removal performance and loading capacity of a novel single-stage nitritation-anammox system with syntrophic micro-granules

Bioresour Technol. 2017 Jul:236:119-128. doi: 10.1016/j.biortech.2017.03.164. Epub 2017 Mar 29.

Authors

Shaopo Wang¹, Yuan Liu², Qigui Niu³, Jiayuan Ji⁴, Toshimasa Hojo⁴, Yu-You Li⁵

Affiliations

¹ School of Environmental and Municipal Engineering, Tianjin Chengjian University, 26# Jinjing Road, Tianjin 300384, China; Tianjin Key Laboratory of Aquatic Science and Technology, 26# Jinjing Road, Tianjin 300384, China.
² Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan; State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
³ School of Environmental Science and Engineering, Shandong University, 27# Shanda South Road, Jinan 250100, China.
⁴ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan.
⁵ Department of Civil and Environmental Engineering, Graduate School of Engineering, Tohoku University, 6-6-06 Aoba, Aramaki, Aoba-ku, Sendai 980-8579, Japan. Electronic address: gyokuyu.ri.a5@tohoku.ac.jp.

PMID: 28399415
DOI: 10.1016/j.biortech.2017.03.164

Abstract

The operation performance of a novel micro-granule based syntrophic system of nitritation and anammox was studied by controlling the oxygen concentration and maintaining a constant temperature of 25°C. With the oxygen concentration of around 0.11 (<0.15)mg/L, the single-stage nitritation-anammox system was startup successfully at a nitrogen loading rate (NLR) of 1.5kgN/m³/d. The reactor was successfully operated at volumetric N loadings ranging from 0.5 to 2.5kgN/m³/d with a high nitrogen removal of 82%. The microbial community was composed by ammonia oxidizing bacteria (AOB) and anammox bacteria forming micro-granules with an average diameter of 0.8mm and good settleability. Results from pyrosequencing analysis revealed that Ca. Kuenenia and Nitrosomonas were selected and enriched in the community over the startup period, and these were identified as the dominant anammox bacteria and AOB species, respectively.

Keywords: Ca. Kuenenia; Nitrogen loading rate; Nitrogen removal; Single-stage nitritation-anammox; Syntrophic micro-granule.

MeSH terms

Bacteria
Bioreactors / microbiology*
Denitrification
Nitrogen
Nitrosomonas

Substances

Nitrogen