Serotonin abrogates dopamine induced aggregation of cytochrome c

Abstract

Importance of cytochrome c arises from its involvement in apoptosis, sequence homology and conserveness during molecular evolution. Dopamine at 25μM concentration for 30h resulted in cytochrome c aggregation as evident by increase in ANS and ThT binding, structural transition from α helix to β sheet and shift in congo red assay. Interestingly, serotonin at 25μM concentration incubated for 60h abrogates the aggregatory effect on cytochrome c. Fibrillation of human cytochrome c in the presence of dopamine gives the clue about conformational changes taking place in protein and example of natural aggregation and inhibitory effect of serotonin gives the clue about natural defence mechanism occurring in human body.

Keywords: Aggregation; Cytochrome c; Dopamine; Serotonin.