Generation and characterization of two iPSC lines from human epicardium-derived cells

Stem Cell Res. 2017 Apr:20:50-53. doi: 10.1016/j.scr.2017.02.007. Epub 2017 Feb 24.

Abstract

Human epicardium-derived cells (EPDC) were reprogrammed to generate two iPSC lines, MCDU1i-EPDC and MCDU2i-EPDC, by nucleofection of episomal-based plasmids expressing the reprogramming factors OCT4, SOX2, KLF4, c-MYC, NANOG and LIN28. Pluripotency was confirmed in vitro by immunofluorescence analysis and embryoid body formation. The iPSC lines and the human embryonic stem cell line H1 show a Pearson correlation co-efficient of 0.951 (MCDU1i-EPDC) and 0.937 (MCDU2i-EPDC) as assessed by comparative transcriptome profiling.

MeSH terms

  • Cell Differentiation
  • Cell Line
  • Cellular Reprogramming*
  • DNA Fingerprinting
  • Embryoid Bodies / metabolism
  • Embryoid Bodies / pathology
  • Female
  • Gene Expression Profiling
  • Human Embryonic Stem Cells / cytology
  • Human Embryonic Stem Cells / metabolism
  • Humans
  • Induced Pluripotent Stem Cells / cytology*
  • Induced Pluripotent Stem Cells / metabolism
  • Karyotype
  • Kruppel-Like Factor 4
  • Microscopy, Fluorescence
  • Pericardium / cytology*
  • Pericardium / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • KLF4 protein, human
  • Kruppel-Like Factor 4
  • Transcription Factors