Malignant canine mammary tumours: Preliminary genomic insights using oligonucleotide array comparative genomic hybridisation analysis

Marta Santos; Patrícia Dias-Pereira; Christina Williams; Carlos Lopes; Matthew Breen

doi:10.1016/j.tvjl.2017.03.005

Malignant canine mammary tumours: Preliminary genomic insights using oligonucleotide array comparative genomic hybridisation analysis

Vet J. 2017 Apr:222:68-71. doi: 10.1016/j.tvjl.2017.03.005. Epub 2017 Mar 28.

Authors

Marta Santos¹, Patrícia Dias-Pereira², Christina Williams³, Carlos Lopes², Matthew Breen⁴

Affiliations

¹ Department of Microscopy, Laboratory of Histology and Embryology, Institute of Biomedical Sciences Abel Salazar, ICBAS - UPorto, University of Porto, Porto, Portugal. Electronic address: mssantos@icbas.up.pt.
² Department of Pathology and Molecular Immunology, ICBAS - UPorto, Porto, Portugal.
³ Department of Molecular Biomedical Sciences, College of Veterinary Medicine North Carolina State University, Raleigh, NC, USA.
⁴ Department of Molecular Biomedical Sciences, College of Veterinary Medicine North Carolina State University, Raleigh, NC, USA; Comparative Medicine Institute, North Carolina State University, Raleigh, NC, USA; Cancer Genetics, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, NC, USA.

PMID: 28392153
DOI: 10.1016/j.tvjl.2017.03.005

Abstract

Neoplastic mammary disease in female dogs represents a major health concern for dog owners and veterinarians, but the genomic basis of the disease is poorly understood. In this study, we performed high resolution oligonucleotide array comparative genomic hybridisation (oaCGH) to assess genome wide DNA copy number changes in 10 malignant canine mammary tumours from seven female dogs, including multiple tumours collected at one time from each of three female dogs. In all but two tumours, genomic imbalances were detected, with losses being more common than gains. Canine chromosomes 9, 22, 26, 27, 34 and X were most frequently affected. Dissimilar oaCGH ratio profiles were observed in multiple tumours from the same dogs, providing preliminary evidence for probable independent pathogenesis. Analysis of adjacent samples of one tumour revealed regional differences in the number of genomic imbalances, suggesting heterogeneity within tumours.

Keywords: Array comparative genomic hybridisation; Canine mammary tumours; Chromosome; Genetics.

MeSH terms

Animals
Comparative Genomic Hybridization / veterinary*
DNA Copy Number Variations
Dog Diseases / genetics*
Dog Diseases / pathology
Dogs
Female
Mammary Neoplasms, Animal / genetics*
Mammary Neoplasms, Animal / pathology