Recent 5-year Findings and Technological Advances in the Proteomic Study of HIV-associated Disorders

Lijun Zhang; Xiaofang Jia; Jun-O Jin; Hongzhou Lu; Zhimi Tan

doi:10.1016/j.gpb.2016.11.002

Recent 5-year Findings and Technological Advances in the Proteomic Study of HIV-associated Disorders

Genomics Proteomics Bioinformatics. 2017 Apr;15(2):110-120. doi: 10.1016/j.gpb.2016.11.002. Epub 2017 Apr 6.

Authors

Lijun Zhang¹, Xiaofang Jia², Jun-O Jin², Hongzhou Lu², Zhimi Tan²

Affiliations

¹ Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China. Electronic address: zhanglijun1221@163.com.
² Shanghai Public Health Clinical Center, Fudan University, Shanghai 201508, China.

Abstract

Human immunodeficiency virus-1 (HIV-1) mainly relies on host factors to complete its life cycle. Hence, it is very important to identify HIV-regulated host proteins. Proteomics is an excellent technique for this purpose because of its high throughput and sensitivity. In this review, we summarized current technological advances in proteomics, including general isobaric tags for relative and absolute quantitation (iTRAQ) and stable isotope labeling by amino acids in cell culture (SILAC), as well as subcellular proteomics and investigation of posttranslational modifications. Furthermore, we reviewed the applications of proteomics in the discovery of HIV-related diseases and HIV infection mechanisms. Proteins identified by proteomic studies might offer new avenues for the diagnosis and treatment of HIV infection and the related diseases.

Keywords: HIV-1; Interaction; Proteomics; SILAC; iTRAQ.

Publication types

Review

MeSH terms

HIV Infections / complications*
HIV Infections / metabolism
HIV Infections / physiopathology
Humans
Proteins / metabolism
Proteomics / methods*

Substances

Proteins