Whole-Genome Sequences of Burkholderia pseudomallei Isolates Exhibiting Decreased Meropenem Susceptibility

Erin P Price; Melissa Laird Smith; Ellen E Paxinos; Luke J Tallon; Lisa Sadzewicz; Naomi Sengamalay; Robert W Baird; Bart J Currie; Derek S Sarovich

doi:10.1128/genomeA.00053-17

Whole-Genome Sequences of Burkholderia pseudomallei Isolates Exhibiting Decreased Meropenem Susceptibility

Genome Announc. 2017 Apr 6;5(14):e00053-17. doi: 10.1128/genomeA.00053-17.

Authors

Erin P Price^{1

2}, Melissa Laird Smith³, Ellen E Paxinos³, Luke J Tallon⁴, Lisa Sadzewicz⁴, Naomi Sengamalay⁴, Robert W Baird^{5

6}, Bart J Currie^{7

5}, Derek S Sarovich^{7

2}

Affiliations

¹ Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia eprice@usc.edu.au.
² Centre for Animal Health Innovation, Faculty of Science, Health, Education and Engineering, University of the Sunshine Coast, Sippy Downs, Queensland, Australia.
³ Pacific Biosciences, Menlo Park, California, USA.
⁴ Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, Maryland, USA.
⁵ Department of Infectious Diseases and Northern Territory Medical Program, Royal Darwin Hospital, Darwin, Northern Territory, Australia.
⁶ Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia.
⁷ Global and Tropical Health Division, Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia.

Abstract

We report here paired isogenic Burkholderia pseudomallei genomes obtained from three patients receiving intravenous meropenem for melioidosis treatment, with post-meropenem isolates developing decreased susceptibility. Two genomes were finished, and four were drafted to improved high-quality standard. These genomes will be used to identify meropenem resistance mechanisms in B. pseudomallei.