Prospects of siRNA applications in regenerative medicine

Fatemeh Mottaghitalab; Ali Rastegari; Mehdi Farokhi; Rassoul Dinarvand; Hossein Hosseinkhani; Keng-Liang Ou; Daniel W Pack; Chuanbin Mao; Meshkat Dinarvand; Yousef Fatahi; Fatemeh Atyabi

doi:10.1016/j.ijpharm.2017.03.092

Prospects of siRNA applications in regenerative medicine

Int J Pharm. 2017 May 30;524(1-2):312-329. doi: 10.1016/j.ijpharm.2017.03.092. Epub 2017 Apr 4.

Authors

Affiliations

¹ Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
² Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
³ National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
⁴ Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
⁵ Innovation Center for Advanced Technology, Matrix, Inc., New York, NY 10029, USA.
⁶ Research Center for Biomedical Devices and Prototyping Production, Research Center for Biomedical Implants and Microsurgery Devices, Taipei Medical University, Taipei, Taiwan.
⁷ Department of Chemical & Materials Engineering and Department of Pharmaceutical Sciences, University of Kentucky, Lexington, KY, United States.
⁸ Department of Chemistry & Biochemistry, Stephenson Life Science Research Center, University of Oklahoma, 101 Stephenson Parkway, Norman, OK 73019, United States; School of Materials Science and Engineering, Zhejiang University, Hangzhou, Zhejiang 310027, China.
⁹ Nanotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Department of Pharmaceutical Nanotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. Electronic address: atyabifa@tums.ac.ir.

PMID: 28385649
DOI: 10.1016/j.ijpharm.2017.03.092

Abstract

Small interfering RNA (siRNA) has established its reputation in the field of tissue engineering owing to its ability to silence the proteins that inhibit tissue regeneration. siRNA is capable of regulating cellular behavior during tissue regeneration processes. The concept of using siRNA technology in regenerative medicine derived from its ability to inhibit the expression of target genes involved in defective tissues and the possibility to induce the expression of tissue-inductive factors that improve the tissue regeneration process. To date, siRNA has been used as a suppressive biomolecule in different tissues, such as nervous tissue, bone, cartilage, heart, kidney, and liver. Moreover, various delivery systems have been applied in order to deliver siRNA to the target tissues. This review will provide an in-depth discussion on the development of siRNA and their delivery systems and mechanisms of action in different tissues.

Keywords: Delivery systems; Gene expression; Tissue engineering; siRNA.

Publication types

Review

MeSH terms

RNA, Small Interfering / pharmacology*
Regenerative Medicine*
Tissue Engineering*

Substances

RNA, Small Interfering