A similar local immune and oxidative stress phenotype in vitiligo and halo nevus

Abstract

Background: Vitiligo and halo nevus are two common T-cell-mediated skin disorders. Although autoimmunity has been suggested to be involved in both diseases, the relationship between vitiligo and halo nevus is not fully understood.

Objective: The aim of the current study was to investigate whether vitiligo and halo nevus share the same immunological and oxidative stress response.

Methods: Infiltrations of T cells, and expressions of chemokine receptors (CXCR3, CCR4, CCR5) and cytotoxic markers (Granzyme B, Perforin) in the lesions of vitiligo and halo nevus were examined by immunohistochemistry. Enzyme-linked immunosorbent assay was performed to analyze the expressions of chemokines in the serum samples and cytotoxic markers secreted by CD8⁺ T cells which were sorted from the peripheral blood mononuclear cells in healthy donors, vitiligo and halo nevus patients. Tissue levels of chemokine receptors and CXCR3 ligands in healthy controls, vitiligo patients and halo nevus patients were determined by qRT-PCR analysis. The percentages of CXCR3⁺ CD4⁺ T and CXCR3⁺ CD8⁺ T cells from the peripheral blood samples were examined by flow cytometry. Tissue and serum hydrogen peroxide (H₂O₂) concentrations were measured using H₂O₂ assay kit.

Results: Immunohistochemistry revealed a significant T-cell response, with pronounced dermal infiltrates of CD8⁺ T cells in vitiligo and halo nevus. The inflammatory cytotoxic markers such as Granzyme B and Perforin were also elevated in vitiligo and halo nevus, suggesting inflammatory responses in situ. By qRT-PCR and ELISA assay, we found significantly increased expressions of the chemokine receptor CXCR3 and its ligands, especially the accumulated CXCL10 in the skin lesions of vitiligo and halo nevus. Moreover, the level of H₂O₂, a key player involved in regulation of the immune response was significantly upregulated in the skin lesions of vitiligo and halo nevus. In addition, the increased H₂O₂ concentration correlated positively with CXCL10 level in skin lesions of vitiligo and halo nevus.

Conclusions: These results demonstrate a H₂O₂-involved autoimmune phenotype in vitiligo and halo nevus, characterized by increased level of IFN-γ-inducible chemokine pair CXCL10-CXCR3, as well as a dense CD8⁺ T infiltration in the skin lesions, thus suggesting a similar pathogenesis of the two diseases.

Keywords: CD8(+) T cells; CXCL10; CXCR3; Halo nevus; Oxidative stress; Vitiligo.