A selective and light-up fluorescent probe for β-galactosidase activity detection and imaging in living cells based on an AIE tetraphenylethylene derivative

Chem Commun (Camb). 2017 Apr 18;53(32):4505-4508. doi: 10.1039/c7cc00249a.

Abstract

An aggregation induced emission (AIE) based bioimaging probe, TPE-Gal, was designed for light-up imaging of β-galactosidase in living cells. The applicability of TPE-Gal in imaging endogenous β-galactosidase activity was confirmed in OVCAR-3 cells.

MeSH terms

  • Cell Line, Tumor
  • Cell Survival
  • Dose-Response Relationship, Drug
  • Female
  • Fluorescent Dyes / analysis
  • Fluorescent Dyes / chemistry*
  • Galactose / pharmacology
  • HeLa Cells
  • Humans
  • Light*
  • Molecular Structure
  • Optical Imaging*
  • Ovarian Neoplasms / diagnostic imaging*
  • Ovarian Neoplasms / drug therapy*
  • Ovarian Neoplasms / metabolism
  • Spectrometry, Fluorescence
  • Stilbenes / chemistry*
  • Structure-Activity Relationship
  • beta-Galactosidase / analysis*
  • beta-Galactosidase / metabolism

Substances

  • Fluorescent Dyes
  • Stilbenes
  • tetraphenylethylene
  • beta-Galactosidase
  • Galactose