Identification and characterization of a human G9P[23] rotavirus strain from a child with diarrhoea in Thailand: evidence for porcine-to-human interspecies transmission

J Gen Virol. 2017 Apr;98(4):532-538. doi: 10.1099/jgv.0.000722. Epub 2017 Apr 6.

Abstract

An unusual rotavirus strain with the G9P[23] genotype (RVA/Human-wt/THA/KKL-117/2014/G9P[23]) was identified in a stool specimen from a 10-month-old child hospitalized with severe diarrhoea. In this study, we sequenced and characterized the complete genome of strain KKL-117. On full-genomic analysis, strain KKL-117 was found to have the following genotype constellation: G9-P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. The non-G/P genotype constellation of this strain (I5-R1-C1-M1-A8-N1-T1-E1-H1) is commonly shared with rotavirus strains from pigs. Furthermore, phylogenetic analysis indicated that each of the 11 genes of strain KKL-117 appeared to be of porcine origin. Our observations provide important insights into the dynamic interactions between human and porcine rotavirus strains.

MeSH terms

  • Animals
  • Cluster Analysis
  • Diarrhea / virology*
  • Feces / virology
  • Genome, Viral
  • Genotype*
  • Humans
  • Infant
  • Phylogeny
  • Rotavirus / classification*
  • Rotavirus / genetics*
  • Rotavirus / isolation & purification
  • Rotavirus Infections / transmission
  • Rotavirus Infections / virology*
  • Sequence Analysis, DNA
  • Sequence Homology
  • Swine
  • Thailand
  • Zoonoses / transmission
  • Zoonoses / virology