Abstract
Phytochemical investigation of the twigs of Syringa oblata var. diatata led to the isolation of two new secoiridoid glucosides, dilatioside A-B (1-2), along with thirteen known ones (3-15). The structures were determined by spectroscopic methods including one and two dimensional (1- and 2D-) NMR techniques, high resolution (HR)-FAB-MS, and chemical methods. The isolated compounds (1-15) were tested for the induction of nerve growth factor (NGF) secretion in a C6 rat glioma cell line and their cytotoxicity against four human cancer cell lines (A549, SK-OV-3, SK-MEL-2, HCT15) in vitro using a sulforhodamine B bioassay. Compounds 5, 7, 8, 10, and 14 were found to induce upregulation of NGF secretion without causing significant cell toxicity.
MeSH terms
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Animals
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Antineoplastic Agents, Phytogenic / chemistry
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Antineoplastic Agents, Phytogenic / isolation & purification
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Antineoplastic Agents, Phytogenic / pharmacology*
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Cell Line, Tumor
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Cell Proliferation / drug effects
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Cell Survival / drug effects
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Dose-Response Relationship, Drug
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Drug Screening Assays, Antitumor
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Humans
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Iridoid Glucosides / chemistry
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Iridoid Glucosides / isolation & purification
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Iridoid Glucosides / pharmacology*
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Molecular Conformation
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Nerve Growth Factors / metabolism
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Neuroprotective Agents / chemistry
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Neuroprotective Agents / isolation & purification
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Neuroprotective Agents / pharmacology*
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Stems / chemistry
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Rats
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Structure-Activity Relationship
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Syringa / chemistry*
Substances
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Antineoplastic Agents, Phytogenic
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Iridoid Glucosides
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Nerve Growth Factors
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Neuroprotective Agents
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Plant Extracts