Objective: To investigate the effect of androgen receptor (AR) on IgG protein expression and the proliferation and migration of prostate cancer cells.
Methods: Western blotting was used to detect the expression of AR protein and IgG in androgen-dependent prostate cancer LNCap cells and castration-resistant prostate cancer PC-3 cells. In AR-overexpressing cells (PC-3-AR cells) established by transfecting PC-3 with AR gene (pCDNA3.1) and LNCap cells with small interfering RNA-mediated AR silencing (LNCap-siAR cells) were analyzed for expressions of AR protein and IgG with Western blotting; the expression of IgG mRNA was detected by Q-PCR, and the cell proliferation and migration were assessed with MTT assay and wound healing assay, respectively.
Results: Compared with PC-3 cells, LNCap cells expressed a higher level of AR protein and a lower level of IgG (P<0.05). PC-3-AR cells showed attenuated proliferation and migration with a lowered expression of IgG (P<0.01), while LNCap-siAR cells showed enhanced proliferation and migration with increased expression of IgG (P<0.01).
Conclusion: The expression of AR is inversely correlated with IgG and is associated with the proliferation and migration of prostate cancer cells in vitro.
目的: 研究前列腺癌细胞中雄激素受体（AR）对免疫球蛋白IgG表达的影响，及对前列腺癌细胞增殖、迁移的作用。
方法: （1）Western blot检测雄激素依赖性前列腺癌细胞株LNCap与去势抵抗性前列腺癌细胞株PC-3中AR、IgG蛋白的表达；（2）AR基因（pCDNA3.1+）转染PC-3构建AR过表达的PC-3-AR细胞，沉默LNCap中AR基因表达构建LNCap-siAR细胞；Western blot检测AR及IgG表达量；Q-PCR检测细胞株中IgG mRNA表达量；四氮甲基唑氮、细胞划痕方法检测细胞的增殖及迁移能力。
结果: LNCap细胞与PC-3细胞相比较，AR高表达，IgG低表达（P＜0.05）。PC-3-AR细胞IgG降低表达（P＜0.01），细胞增殖及迁移能力减弱；LNCap-siAR细胞IgG增高表达（P＜0.01），细胞增殖及迁移能力增强。
结论: AR调控IgG蛋白表达呈负相关并与前列腺癌细胞增殖、迁移能力相关。