Abstract
The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.
Publication types
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Comparative Study
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Evaluation Study
MeSH terms
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Biomarkers, Tumor / analysis*
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Biosensing Techniques / methods*
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Biosensing Techniques / statistics & numerical data
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Cell Line, Tumor
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Electrochemical Techniques
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Enzyme-Linked Immunosorbent Assay
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Fibroblast Growth Factor 4 / analysis*
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Humans
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Immunoassay / methods
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Limit of Detection
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MCF-7 Cells
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Magnetics
Substances
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Biomarkers, Tumor
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FGF4 protein, human
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Fibroblast Growth Factor 4
Grants and funding
The financial support of the Spanish Ministerio de Economía y Competitividad Research Projects, CTQ2015-64402-C2-1-R and SAF2014-53209-R, and the NANOAVANSENS Program from the Comunidad de Madrid (S2013/MT-3029) are gratefully acknowledged. R.M. Torrente-Rodríguez and V. Ruiz-Valdepeñas Montiel acknowledge predoctoral contracts from the Spanish Ministerio de Economía y Competitividad and Universidad Complutense de Madrid, respectively. Rodrigo Barderas is supported by the Ramón y Cajal Program of the MINECO. María Garranzo-Asensio is supported by a contract of the Programa Operativo de Empleo Juvenil y la Iniciativa de Empleo Juvenil (YEI) with the participation of the Consejería de Educación, Juventud y Deporte de la Comunidad de Madrid and of the Fondo Social Europeo. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.