Molecular Characterization of Escherichia coli Isolates Carrying mcr-1, fosA3, and Extended-Spectrum-β-Lactamase Genes from Food Samples in China

Xiaobo Liu; Ruichao Li; Zhiwei Zheng; Kaichao Chen; Miaomiao Xie; Edward Wai-Chi Chan; Shu Geng; Sheng Chen

doi:10.1128/AAC.00064-17

Molecular Characterization of Escherichia coli Isolates Carrying mcr-1, fosA3, and Extended-Spectrum-β-Lactamase Genes from Food Samples in China

Antimicrob Agents Chemother. 2017 May 24;61(6):e00064-17. doi: 10.1128/AAC.00064-17. Print 2017 Jun.

Authors

Xiaobo Liu^{1

2}, Ruichao Li^{2

3}, Zhiwei Zheng^{1

2}, Kaichao Chen^{2

3}, Miaomiao Xie^{2

3}, Edward Wai-Chi Chan³, Shu Geng⁴, Sheng Chen^{5

3}

Affiliations

¹ College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China.
² Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China.
³ The State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, SAR.
⁴ College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi, China sgeng@ucdavis.edu sheng.chen@polyu.edu.hk.
⁵ Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shenzhen Research Institute, Shenzhen, China sgeng@ucdavis.edu sheng.chen@polyu.edu.hk.

Abstract

This study surveyed the prevalence of mcr-1 in extended-spectrum-β-lactamase (ESBL)-producing Escherichia coli strains of food origin in China and identified strains that carried mcr-1, fosA3, and ESBL genes, which were carried in various plasmids. The mcr-1 and ESBL genes could be cotransferred by one or more types of plasmids. The presence of these multidrug-resistant E. coli strains in food products might pose a huge threat to public health.

Keywords: ESBL-producing E. coli; Tn6330; circular intermediate; fosA3; mcr-1; plasmids; transmission.

MeSH terms

Anti-Bacterial Agents / pharmacology
China
DNA, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics
Escherichia coli / drug effects*
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Proteins / genetics*
Food Contamination
Foodborne Diseases / microbiology
Genome, Bacterial / genetics
Microbial Sensitivity Tests
Plasmids / genetics
Red Meat / microbiology
Seafood / microbiology
beta-Lactamases / genetics*
beta-Lactams / pharmacology

Substances

Anti-Bacterial Agents
DNA, Bacterial
Escherichia coli Proteins
FosA(3) protein, E coli
MCR-1 protein, E coli
beta-Lactams
beta-Lactamases