Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli

Roshan Jha; Hye Young Cho; Ameeq Ul Mushtaq; Kiho Lee; Dae Gyu Kim; Sunghoon Kim; Young Ho Jeon

doi:10.1016/j.dib.2017.03.011

Data on optimization of expression and purification of AIMP2-DX2 protein in Escherichia coli

Data Brief. 2017 Mar 10:11:533-536. doi: 10.1016/j.dib.2017.03.011. eCollection 2017 Apr.

Authors

Roshan Jha¹, Hye Young Cho¹, Ameeq Ul Mushtaq¹, Kiho Lee¹, Dae Gyu Kim², Sunghoon Kim², Young Ho Jeon¹

Affiliations

¹ College of Pharmacy, Korea University, 2511 Sejong-ro, Sejong 30019, Republic of Korea.
² Medicinal Bioconvergence Research Center, Seoul National University, Seoul 08826, Republic of Korea; Research Institute of Pharmaceutical Sciences, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.

Abstract

AIMP2-DX2 is a splicing variant of AIMP2 protein which has been implicated in human lung cancer and chemoresistance of ovarian cancer (J.W. Choi, D.G. Kim, A.E. Lee, H.R. Kim, J.Y. Lee, N.H. Kwon, et al., 2011; J.W. Choi, J.W. Lee, J.K. Kim, H.K. Jeon, J.J. Choi, D.G. Kim, et al., 2012) [1,2]. We have shown, here, the data for the expression of AIMP2-DX2 protein in Escherichia coli and optimization of the critical steps in purification of AIMP2-DX2. The data described here has been successfully used to get a maximum yield of highly pure AIMP2-DX2 for subsequent characterization of its biophysical property in: "Purification and biophysical characterization of the AIMP2-DX2 protein" (R. Jha, H.Y. Cho, A. Ul Mushtaq, K. Lee, D.G. Kim, S. Kim, et al., 2017) [3].

Keywords: AIMP2; AIMP2, Aminoacyl-tRNA synthetase interacting multifunctional protein 2; AIMP2-DX2; AIMP2-DX2 or DX2, splicing variant of AIMP2 lacking exon 2; DTT, dithiorietol; Expression and solubility; IPTG, isopropyl β-D-1-thiogalactopyranoside; Protein stabilizers; SDS-PAGE, Sodium dodecyl sulfate polyacrylamide gel electrophoresis; SUMO, Small Ubiquitin-like modifier; SUMO-tag.