Improved xylitol production by expressing a novel d-arabitol dehydrogenase from isolated Gluconobacter sp. JX-05 and co-biotransformation of whole cells

Abstract

In the present study, a novel ardh gene encoding d-arabitol dehydrogenase (ArDH) was cloned and expressed in Escherichia coli from a new isolated strain of Gluconobacter sp. JX-05. Sequence analysis revealed that ArDH containing a NAD(P)-binding motif and a classical active site motif belongs to the short-chain dehydrogenase family. Subsequently, the optimal pH and temperature, specific activities and kinetic parameter of ArDH were determined. In the co-biotransformation by the whole cells of BL21-ardh and BL21-xdh, 26.1g/L xylitol was produced from 30g/L d-arabitol in 22h with a yield of 0.87g/g. The xylitol production was increased by more than two times as compared with that of Gluconobacter sp. alone, and was improved 10.1% than that of Gluconobacter sp. mixed with BL21-xdh.

Keywords: Biotransformation; Characteristic; Gluconobacter sp.; Whole cell; d-Arabitol dehydrogenase.