Regulation of Isoform Expression by Blocking Polyadenylation Signal Sequences with Morpholinos

Methods Mol Biol. 2017:1565:141-150. doi: 10.1007/978-1-4939-6817-6_12.

Abstract

Alternative polyadenylation is increasingly being recognized as an important layer of gene regulation. Antisense-mediated modulation of alternative polyadenylation represents an attractive strategy for the regulation of gene expression as well as potential therapeutic applications. In this chapter, we describe methods to upregulate the functional Kv11.1 isoform expression by blocking intronic polyadenylation signal sequences with antisense morpholinos.

Keywords: Alternative polyadenylation; KCNH2; Long QT syndrome; Morpholino; Potassium channel; hERG.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Alternative Splicing*
  • ERG1 Potassium Channel / genetics
  • Ether-A-Go-Go Potassium Channels / genetics
  • Gene Expression Regulation*
  • Gene Order
  • HEK293 Cells
  • Humans
  • Long QT Syndrome / genetics
  • Morpholinos / genetics*
  • Poly A*
  • Polyadenylation*
  • Protein Isoforms / genetics
  • Transfection

Substances

  • ERG1 Potassium Channel
  • Ether-A-Go-Go Potassium Channels
  • KCNH2 protein, human
  • Morpholinos
  • Protein Isoforms
  • Poly A