Abstract
Here, we investigated the effects of dual delivery of IGF-1R siRNA and doxorubicin by chitosan nanoparticles on viability of A549 lung cancer cells line by utilization of MTT and qRT-PCR. Furthermore apoptosis and migration of treated cells were assessed by Annexin-PI and wound healing assays, respectively. The chitosan nanoparticles had about 176 nm size with zeta potential and polydispersive index about 11 mV and 0.3, respectively. The IGF-1R siRNA had synergistic effect on DOX-induced cytotoxicity and apoptosis in tumour cells. In addition, siRNA/DOX-loaded chitosan nanoparticles could significantly decrease migration and expressions of mmp9, VEGF and STAT3 in A549 cells.
Keywords:
Chitosan nanoparticle; IGF-1R; doxorubicin; lung cancer; ss siRNA.
MeSH terms
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A549 Cells
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Apoptosis / drug effects
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Apoptosis / genetics
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Cell Movement / drug effects
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Cell Movement / genetics
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Chitosan / chemistry*
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Down-Regulation / drug effects
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Down-Regulation / genetics
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Doxorubicin / chemistry*
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Doxorubicin / pharmacology
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Drug Carriers / chemistry
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Drug Liberation
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Humans
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Hydrogen-Ion Concentration
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Lung Neoplasms / pathology*
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Matrix Metalloproteinase 9 / genetics
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Nanoparticles / chemistry*
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Neoplasm Invasiveness
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RNA, Small Interfering / chemistry*
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RNA, Small Interfering / genetics
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Receptor, IGF Type 1 / deficiency*
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Receptor, IGF Type 1 / genetics*
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STAT3 Transcription Factor / genetics
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Vascular Endothelial Growth Factor A / genetics
Substances
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Drug Carriers
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RNA, Small Interfering
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STAT3 Transcription Factor
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Vascular Endothelial Growth Factor A
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Doxorubicin
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Chitosan
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Receptor, IGF Type 1
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Matrix Metalloproteinase 9