Association of Glu298Asp Polymorphism of Endothelial NO Synthase Gene with Metabolic Syndrome Development: a Pilot Study

Bull Exp Biol Med. 2017 Mar;162(5):615-618. doi: 10.1007/s10517-017-3670-9. Epub 2017 Mar 30.

Abstract

We studied association of single nucleotide polymorphism Glu298Asp (rs1799983) of the NOS3 gene with the risk of metabolic syndrome in the Slavic population. Blood samples were obtained from 128 patients with metabolic syndrome and 100 healthy individuals. Polymorphism Glu298Asp of the NOS3 gene was genotyped by allele-specific PCR. Allele Asp (OR=1.95, 95%CI 1.29-2.95, p=0.007) and genotype Asp/Asp (OR=2.56, 95%CI 0.98-6.72, p=0.04) were associated with the risk of metabolic syndrome in Slavic population. Patients with metabolic syndrome carrying genotype Asp/Asp had higher serum endothelin-1 level in comparison with Glu/Asp and Glu/Glu carriers.

Keywords: endothelial NO synthase; endothelial dysfunction; metabolic syndrome; nitric oxide; single nucleotide polymorphism.

MeSH terms

  • Adult
  • Case-Control Studies
  • Endothelin-1 / blood
  • Female
  • Gene Frequency
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Humans
  • Male
  • Metabolic Syndrome / blood
  • Metabolic Syndrome / enzymology
  • Metabolic Syndrome / genetics*
  • Middle Aged
  • Nitric Oxide Synthase Type III / genetics*
  • Pilot Projects
  • Polymorphism, Single Nucleotide

Substances

  • Endothelin-1
  • NOS3 protein, human
  • Nitric Oxide Synthase Type III