Abstract
A phytochemical study on the whole plant of Spermacoce latifolia led to the isolation of a new anthraquinone, 1,2,6-trihydroxy-5-methoxy-9,10-anthraquinone (1), and a new naphthoquinone, (2R)-6-hydroxy-7-methoxy-dehydroiso-α-lapachone (2), together with three known anthraquinones (3-5). Their structures were established on the basis of detailed spectroscopic analysis, including one- and two-dimensional NMR, ESI-MS, and HR-ESI-MS techniques. All the compounds were isolated from S. latifolia for the first time. Compounds 1, 2, 4, and 5 showed significant antibacterial activity toward Bacillus subtilis with MIC values ranging from 0.9 to 31.2 μg/ml, and compound 4 aslo exhibited antibacterial activity against Bacillus cereus with a MIC value 62.5 μg/ml. Compound 1 was further revealed to show significant in vitro α-glucosidase inhibitory activity with IC50 value of 0.653 mM.
Keywords:
Rubiaceae; Spermacoce latifolia; anthraquinone; antibacterial activity; naphthoquinone; α-glucosidase inhibitor.
MeSH terms
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Anthraquinones / chemistry
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Anthraquinones / isolation & purification*
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Anthraquinones / pharmacology
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / isolation & purification*
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Anti-Bacterial Agents / pharmacology
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Bacillus cereus / drug effects
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Bacillus subtilis / drug effects
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Drugs, Chinese Herbal / chemistry
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Drugs, Chinese Herbal / isolation & purification*
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Drugs, Chinese Herbal / pharmacology
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Escherichia coli / drug effects
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Glycoside Hydrolase Inhibitors / chemistry
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Glycoside Hydrolase Inhibitors / isolation & purification*
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Glycoside Hydrolase Inhibitors / pharmacology
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Inhibitory Concentration 50
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Methicillin-Resistant Staphylococcus aureus / drug effects
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Microbial Sensitivity Tests
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Molecular Structure
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Naphthoquinones / chemistry
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Naphthoquinones / isolation & purification*
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Naphthoquinones / pharmacology
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Rubiaceae / chemistry*
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Salmonella typhimurium / drug effects
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Shigella dysenteriae / drug effects
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alpha-Glucosidases / drug effects
Substances
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1,2,6-trihydroxy-5-methoxy-9,10-anthraquinone
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Anthraquinones
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Anti-Bacterial Agents
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Drugs, Chinese Herbal
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Glycoside Hydrolase Inhibitors
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Naphthoquinones
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alpha-Glucosidases