Objective: To explore the effects of BPA on the expression of N-cadherin, Vimentin and FSHR in rat Sertoli cells. Methods: Primary Sertoli cells collected from prepuberty rats (18-21 d) were cultured for 48 h, and then they were treated with 0, 30, 50, 70 μmol/L BPA respectively for 24 h. The methods of MTT, real-time quantitative PCR and Western blotting were utilized to measure the cell ability of Sertoli cells, the mRNA and protein expression levels of N-cadherin, Vimentin and FSHR respectively. Results: Compared with control, the cell abilities of Sertoli cells in 50 μmol/L BPA group and 70 μmol/L BPA group increased significantly (P<0.05) . The cell abilities of Sertoli cells decreased with the increases of exposure doses of BPA. Compared with control, the expression of N-cadherin mRNA only increased in 30 μmol/L BPA group (P<0.05) , the expression of Vimentin mRNA decreased significantly in all doses group of BPA (P<0.05) , the expression of FSHR mRNA increased in all doses group of BPA (P<0.05) . Compared with the control, the protein levels of N-cadherin increased significantly in 50 μmol/L BPA group (P<0.05) , the protein levels of Vimentin decreased significantly in all doses group of BPA (P<0.05) , the protein levels of FSHR decreased significantly in 50 μmol/L BPA group and 70 μmol/L BPA group (P<0.05) . Conclusion: The mechanism of testicular toxicity from BPA might be the alterations of N-cadherin, Vimentin and FSHR by disturbing normal spermatogenesis.
目的： 研究双酚A（BPA）对离体培养青春前期大鼠睾丸支持细胞N-钙黏蛋白（N-cadherin）、波形蛋白（Vimentin）和卵泡刺激素受体（FSHR）表达的影响。 方法： 取3只健康18~21日龄SPF级SD雄性大鼠进行睾丸支持细胞原代培养，48 h后分别暴露于含终浓度为0（溶剂对照）、30、50、70 μmol/L BPA的培养基中染毒24 h。采用MTT法检测支持细胞的细胞活性，分别采用实时荧光定量PCR（real-time quantitative PCR）法和Western blotting法检测支持细胞N-cadherin、Vimentin及FSHR mRNA和蛋白的表达水平。 结果： 与溶剂对照组相比，50、70 μmol/L BPA染毒组睾丸支持细胞的存活率均下降，差异有统计学意义（P<0.05）；且随着BPA染毒剂量的升高，睾丸支持细胞的存活率呈逐渐下降的趋势。与溶剂对照组相比，各剂量BPA染毒组睾丸支持细胞FSHR mRNA的表达水平均较高，而Vimentin mRNA的表达水平均较低，仅30 μmol/L BPA染毒组睾丸支持细胞N-cadherin mRNA的表达水平升高，差异均有统计学意义（P<0.05）。与溶剂对照组比较，各剂量BPA染毒组睾丸支持细胞Vimentin蛋白及50、70 μmol/L BPA染毒组睾丸支持细胞FSHR蛋白的表达水平均较低，仅50 μmol/L BPA染毒组睾丸支持细胞N-cadherin蛋白的表达水平升高，差异均有统计学意义（P<0.05）。 结论： BPA通过干扰支持细胞内N-cadherin、Vimentin和FSHR蛋白的表达来影响正常的精子发生，从而产生睾丸毒性。.
Keywords: Bisphenol A; FSHR; N-cadherin; Sertoli cells; Vimentin.