SILAC Mass Spectrometry Profiling: A Psychiatric Disorder Perspective

Adv Exp Med Biol. 2017:974:289-298. doi: 10.1007/978-3-319-52479-5_27.

Abstract

Stable isotope labelling by amino acids in cell culture (SILAC) is a technique that allows proteomic profiling of cells. In this chapter we describe a protocol for the identification and quantification of newly synthesised proteins. The methodology can be applied to any cultured cell system with relevance to schizophrenia, affective disorders and autism spectrum conditions including those addressing responses to pharmacological stimuli.

Keywords: Cell culture; Drug responses; Proteomics; Psychiatric disorders; Stable isotope labelling by amino acids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carbon Isotopes
  • Cell Fractionation
  • Cells, Cultured
  • Chromatography, High Pressure Liquid / methods
  • Chromatography, Liquid / methods
  • Electrophoresis, Polyacrylamide Gel / methods
  • Humans
  • Isotope Labeling / methods*
  • Lysine / metabolism
  • Mental Disorders / metabolism*
  • Nerve Tissue Proteins / analysis*
  • Nerve Tissue Proteins / biosynthesis
  • Nitrogen Isotopes
  • Peptide Fragments / analysis
  • Proteomics / methods*
  • Tandem Mass Spectrometry / methods*

Substances

  • Carbon Isotopes
  • Nerve Tissue Proteins
  • Nitrogen Isotopes
  • Peptide Fragments
  • Lysine