Abstract
HutZ is a heme-degrading enzyme. We found that the heme-degradation reaction by HutZ is inhibited by the iron chelators. Kinetic analysis of each heme-degradation step suggests that water molecules hydrogen bonded to Thr27 are involved in proton transfer to Fe(iii)-OO-, and that this step is inhibited by iron chelators.
MeSH terms
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Bacterial Proteins / antagonists & inhibitors*
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Ferric Compounds / chemistry
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Heme / chemistry
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Heme / metabolism*
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Hydrogen / chemistry
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Iron / chemistry
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Iron / metabolism
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Iron Chelating Agents / pharmacology*
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Kinetics
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Threonine / chemistry
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Vibrio cholerae / enzymology*
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Water / chemistry
Substances
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Bacterial Proteins
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Ferric Compounds
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Iron Chelating Agents
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Water
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ferric oxide
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Threonine
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Heme
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Hydrogen
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Iron