Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli

Chian-Hui Lai; Syer Choon Lim; Liang-Chun Wu; Chien-Fang Wang; Wen-Sy Tsai; Han-Chung Wu; Ying-Chih Chang

doi:10.1039/c7cc00247e

Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli

Chem Commun (Camb). 2017 Apr 6;53(29):4152-4155. doi: 10.1039/c7cc00247e.

Authors

Chian-Hui Lai¹, Syer Choon Lim², Liang-Chun Wu², Chien-Fang Wang², Wen-Sy Tsai³, Han-Chung Wu⁴, Ying-Chih Chang⁵

Affiliations

¹ Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw and Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan.
² Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw.
³ Graduate Institute of Clinical Medical Science, Chang-Gung University, Taoyuan, Taiwan and Division of Colon and Rectal Surgery, Chang-Gung Memorial Hospital, Taoyuan, Taiwan.
⁴ Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan.
⁵ Genomics Research Center, Academia Sinica, Taipei, Taiwan. yingchih@gate.sinica.edu.tw and Department of Chemical Engineering, Stanford University, Stanford, CA 94305, USA.

PMID: 28352905
DOI: 10.1039/c7cc00247e

Abstract

We design and synthesize EpCAM antibodies with Fc-domain site-specific linkers that allow preferential alignment when coated on microfluidic devices for capturing circulating tumor cells (CTCs) from colorectal cancer patients. The aligned coating is shown to increase the capture efficiency of CTCs and microemboli by 1.6 and 3.0-fold, respectively (both P < 0.05).

MeSH terms

Antibodies, Immobilized / chemistry*
Antibodies, Immobilized / immunology*
Cell Separation / methods*
Colorectal Neoplasms / immunology
Colorectal Neoplasms / pathology*
Enzyme-Linked Immunosorbent Assay
Humans
Intracranial Embolism / immunology
Intracranial Embolism / pathology*
Microfluidic Analytical Techniques*
Molecular Conformation
Neoplastic Cells, Circulating / immunology
Neoplastic Cells, Circulating / pathology*

Substances

Antibodies, Immobilized