Bioimprinting as a tool for the detection of aflatoxin B1 using a capacitive biosensor

Alvaro V Gutierrez R; Martin Hedström; Bo Mattiasson

doi:10.1016/j.btre.2016.05.006

Bioimprinting as a tool for the detection of aflatoxin B1 using a capacitive biosensor

Biotechnol Rep (Amst). 2016 May 25:11:12-17. doi: 10.1016/j.btre.2016.05.006. eCollection 2016 Sep.

Authors

Alvaro V Gutierrez R¹, Martin Hedström², Bo Mattiasson²

Affiliations

¹ Division of Biotechnology, Lund University, Lund, Sweden; IIFB, FCFB, Universidad Mayor de San Andres, La Paz, Bolivia.
² Division of Biotechnology, Lund University, Lund, Sweden; CapSenze Biosystems AB, Scheelevägen 22, Lund, Sweden.

Abstract

A strategy for the detection of aflatoxin B1 using a capacitive biosensor has been studied. The use of proteins for the generation of sites with high specificity against aflatoxin B1 are produced via bioimprinting. This technique has become a tool for the detection of aflatoxin B1 using a capacitive biosensor. The results demonstrate the ability to generate specific interactions with aflatoxin B1 with a linear relation between signals registered and log concentration of the target aflatoxin in the concentration range of 3.2 × 10^-6 to 3.2 × 10^-9 M when using ovalbumin as framework for the bioimprinting.

Keywords: Aflatoxin; Bioimprinting; Capacitance; Imprinted protein.