Drosophila Male Meiosis

Silvia Bonaccorsi; Maurizio Gatti

doi:10.1007/978-1-4939-6340-9_16

Drosophila Male Meiosis

Methods Mol Biol. 2017:1471:277-288. doi: 10.1007/978-1-4939-6340-9_16.

Authors

Silvia Bonaccorsi¹, Maurizio Gatti^{2

3}

Affiliations

¹ Department of Biology and Biotechnology "Charles Darwin", Sapienza, University of Rome, P.le. A Moro 5, 00185, Rome, Italy.
² Department of Biology and Biotechnology "Charles Darwin", Sapienza, University of Rome, P.le. A Moro 5, 00185, Rome, Italy. maurizio.gatti@uniroma1.it.
³ Institute of Molecular Biology and Pathology (IBPM) of CNR, Rome, Italy. maurizio.gatti@uniroma1.it.

PMID: 28349403
DOI: 10.1007/978-1-4939-6340-9_16

Abstract

In Drosophila males, there is no synaptonemal complex and recombination does not occur. Thus, Drosophila male meiosis is a good model system for the analysis of achiasmate chromosome segregation. In addition, due to their large size, the meiotic spindles of Drosophila males are an excellent system for mutational dissection of the mechanisms of spindle assembly. Here, we describe the main techniques for visualization of live Drosophila testes and for preparation of fixed meiotic chromosomes and spindles.

Keywords: Chromosomes; Drosophila; Immunostaining; Live analysis; Male meiosis; Meiotic spindle; Spermatogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chromosomes, Insect*
Cytological Techniques / instrumentation
Cytological Techniques / methods
Drosophila / cytology*
Drosophila / genetics
Male
Meiosis*
Spindle Apparatus
Testis / cytology*