Accurate Size and Size-Distribution Determination of Polystyrene Latex Nanoparticles in Aqueous Medium Using Dynamic Light Scattering and Asymmetrical Flow Field Flow Fractionation with Multi-Angle Light Scattering

Haruhisa Kato; Ayako Nakamura; Kayori Takahashi; Shinichi Kinugasa

doi:10.3390/nano2010015

Accurate Size and Size-Distribution Determination of Polystyrene Latex Nanoparticles in Aqueous Medium Using Dynamic Light Scattering and Asymmetrical Flow Field Flow Fractionation with Multi-Angle Light Scattering

Nanomaterials (Basel). 2012 Jan 5;2(1):15-30. doi: 10.3390/nano2010015.

Authors

Haruhisa Kato¹, Ayako Nakamura², Kayori Takahashi³, Shinichi Kinugasa⁴

Affiliations

¹ Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. H-kato@aist.go.jp.
² Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. nakamura@tasc-nt.or.jp.
³ Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. kayori.takahashi@ni.aist.go.jp.
⁴ Polymer Standards Section Japan (PSSJ), Particle Measurement Section (PMS), National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan. s.kinugasa@aist.go.jp.

Abstract

Accurate determination of the intensity-average diameter of polystyrene latex (PS-latex) by dynamic light scattering (DLS) was carried out through extrapolation of both the concentration of PS-latex and the observed scattering angle. Intensity-average diameter and size distribution were reliably determined by asymmetric flow field flow fractionation (AFFFF) using multi-angle light scattering (MALS) with consideration of band broadening in AFFFF separation. The intensity-average diameter determined by DLS and AFFFF-MALS agreed well within the estimated uncertainties, although the size distribution of PS-latex determined by DLS was less reliable in comparison with that determined by AFFFF-MALS.

Keywords: asymmetric flow field flow fractionation; dynamic light scattering; multi-angle light scattering; nanoparticle; polystyrene latex.