Development of an isothermal recombinase polymerase amplification assay for rapid detection of pseudorabies virus

Mol Cell Probes. 2017 Jun:33:32-35. doi: 10.1016/j.mcp.2017.03.005. Epub 2017 Mar 22.

Abstract

Recombinase polymerase amplification assays using real-time fluorescent detection (real-time RPA assay) and lateral flow dipstick (RPA LFD assay) were developed targeting the gD gene of pseudorabies virus (PRV). Both assays were performed at 39 °C within 20 min. The sensitivity of the real-time RPA assay and the RPA LFD assay was 100 copies per reaction and 160 copies per reaction, respectively. Both assays did not detect DNAs from other virus or PRV negative samples. Therefore, the developed RPA assays provide a rapid, simple, sensitive and specific alternative tool for detection of PRV.

Keywords: PRV; RPA LFD assay; Real-time RPA assay; Recombinase polymerase amplification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Herpesvirus 1, Suid / genetics
  • Herpesvirus 1, Suid / isolation & purification*
  • Herpesvirus 1, Suid / pathogenicity
  • Nucleic Acid Amplification Techniques / methods
  • Polymerase Chain Reaction / methods
  • Pseudorabies / diagnosis*
  • Pseudorabies / genetics
  • Pseudorabies / virology
  • Recombinases / genetics*
  • Swine / virology

Substances

  • Recombinases