Quinine bonded to superficially porous particles for high-efficiency and ultrafast liquid and supercritical fluid chromatography

Darshan C Patel; Zachary S Breitbach; JeongJae Yu; Kate A Nguyen; Daniel W Armstrong

doi:10.1016/j.aca.2017.02.005

Quinine bonded to superficially porous particles for high-efficiency and ultrafast liquid and supercritical fluid chromatography

Anal Chim Acta. 2017 Apr 22:963:164-174. doi: 10.1016/j.aca.2017.02.005. Epub 2017 Feb 20.

Authors

Darshan C Patel¹, Zachary S Breitbach¹, JeongJae Yu², Kate A Nguyen¹, Daniel W Armstrong³

Affiliations

¹ Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, United States.
² Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, United States; Department of Chemistry, Kyungpook National University, Daegu, 702-701, Republic of Korea.
³ Department of Chemistry and Biochemistry, University of Texas at Arlington, 700 Planetarium Place, Arlington, TX 76019, United States. Electronic address: sec4dwa@uta.edu.

PMID: 28335970
DOI: 10.1016/j.aca.2017.02.005

Abstract

Two new anion-exchange columns were prepared by bonding tert-butyl carbamoylated quinine to 2.7 μm superficially porous particle (SPP) silica to create chiral stationary phases for high-efficiency and ultrafast chromatography. Performance and retention parameters of these new columns are compared with an analogous 5 μm fully porous particle (FPP) based Chiralpak QNAX column and a 3-4 fold increase in efficiency was observed. Ultrafast separations ranging from 12 s down to sub-second are shown using 2.7 μm SPPs bonded via hydrosilation to the selector. Potential benefits of 2.7 μm SPP based columns for increased LC-MS compatibility were investigated. A van Deemter plot comparison showed 2.7 μm SPP based columns provided a lower reduced plate height and a higher optimal linear velocity compared to the 5 μm FPP based column. With geometry-independent kinetic plots, 2.7 μm SPP and 5 μm FPP based columns were assessed for their kinetic performance and the maximal number of plates each column can generate in a given analysis time. The 2.7 μm SPP based column showed remarkable performance improvements in speed and efficiency as indicated by the kinetic plots.

Keywords: Chiral separations; High-efficiency chiral stationary phase; Kinetic plots; Sub/supercritical fluid chromatography (SFC); Superficially porous particles; Ultra-high performance liquid chromatography (UHPLC).