Electrochemical Synthesis of Polypyrrole, Reduced Graphene Oxide, and Gold Nanoparticles Composite and Its Application to Hydrogen Peroxide Biosensor

Baoyan Wu; Na Zhao; Shihua Hou; Cong Zhang

doi:10.3390/nano6110220

Electrochemical Synthesis of Polypyrrole, Reduced Graphene Oxide, and Gold Nanoparticles Composite and Its Application to Hydrogen Peroxide Biosensor

Nanomaterials (Basel). 2016 Nov 21;6(11):220. doi: 10.3390/nano6110220.

Authors

Baoyan Wu¹, Na Zhao², Shihua Hou³, Cong Zhang⁴

Affiliations

¹ MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. wubaoyan@scnu.edu.cn.
² MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. zhaona5251@163.com.
³ School of Electronic and Information Engineering, South China University of Technology, Guangzhou 510640, China. shhou@scut.edu.cn.
⁴ The Key Laboratory of Bioactive Materials Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China. zhangcong0672@163.com.

Abstract

Here we report a facile eco-friendly one-step electrochemical approach for the fabrication of a polypyrrole (PPy), reduced graphene oxide (RGO), and gold nanoparticles (nanoAu) biocomposite on a glassy carbon electrode (GCE). The electrochemical behaviors of PPy-RGO-nanoAu and its application to electrochemical detection of hydrogen peroxide were investigated by cyclic voltammetry. Graphene oxide and pyrrole monomer were first mixed and casted on the surface of a cleaned GCE. After an electrochemical processing consisting of the electrooxidation of pyrrole monomer and simultaneous electroreduction of graphene oxide and auric ions (Au³⁺) in aqueous solution, a PPy-RGO-nanoAu biocomposite was synthesized on GCE. Each component of PPy-RGO-nanoAu is electroactive without non-electroactive substance. The obtained PPy-RGO-nanoAu/GCE exhibited high electrocatalytic activity toward hydrogen peroxide, which allows the detection of hydrogen peroxide at a negative potential of about -0.62 V vs. SCE. The amperometric responses of the biosensor displayed a sensitivity of 40 µA/mM, a linear range of 32 µM-2 mM, and a detection limit of 2.7 µM (signal-to-noise ratio = 3) with good stability and acceptable reproducibility and selectivity. The results clearly demonstrate the potential of the as-prepared PPy-RGO-nanoAu biocomposite for use as a highly electroactive matrix for an amperometric biosensor.

Keywords: electrodeposition; gold nanoparticles; hydrogen peroxide biosensor; polypyrrole; reduced graphene oxide.