Potential application of waste from castor bean (Ricinus communis L.) for production for xylanase of interest in the industry

Polyanna Nunes Herculano; Keila Aparecida Moreira; Raquel Pedrosa Bezerra; Tatiana Souza Porto; Cristina Maria de Souza-Motta; Ana Lúcia Figueiredo Porto

doi:10.1007/s13205-016-0463-1

Potential application of waste from castor bean (Ricinus communis L.) for production for xylanase of interest in the industry

3 Biotech. 2016 Dec;6(2):144. doi: 10.1007/s13205-016-0463-1. Epub 2016 Jun 23.

Authors

Polyanna Nunes Herculano^{1

2}, Keila Aparecida Moreira³, Raquel Pedrosa Bezerra⁴, Tatiana Souza Porto³, Cristina Maria de Souza-Motta¹, Ana Lúcia Figueiredo Porto²

Affiliations

¹ Mycology Department, Federal University of Pernambuco, Recife, PE, Brazil.
² Animal Morphology and Physiology Department, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s/n, Recife, PE, 52171-900, Brazil.
³ Academic Unit of Garanhuns, Federal Rural University of Pernambuco, Garanhuns, PE, Brazil.
⁴ Animal Morphology and Physiology Department, Federal Rural University of Pernambuco, Av. Dom Manoel de Medeiros s/n, Recife, PE, 52171-900, Brazil. rpbezerra@yahoo.com.br.

Abstract

Xylanases activity (XY) from Aspergillus japonicus URM5620 produced by Solid-State Fermentation (SSF) of castor press cake (Ricinus communis) on different conditions of production and extraction by PEG/citrate aqueous two-phase system (ATPS) were investigated. XY production was influenced by substrate amount (5-10 g), initial moisture (15-35 %), pH (4.0-6.0) and temperature (25-35 °C), obtaining the maximum activity of 29,085 ± 1808 U g ds^-1 using 5.0 g of substrate with initial moisture of 15 % at 25 °C and pH 6.0, after 120 h of fermentation. The influence of PEG molar mass (1000-8000 g mol^-1), phase concentrations (PEG 20.0-24.0 % w/w and sodium citrate 15-20 % w/w) and pH (6.0-8.0) on partition coefficient, purification factor, yield and selectivity of XY were determinate. Enzyme partitioning into the PEG rich phase was favored by M _PEG 8000 (g mol^-1), C _PEG 24 % (w/w), C _C 20 % (w/w) and pH 8.0, resulting in partition coefficient of 50.78, activity yield of 268 %, 7.20-fold purification factor and selectivity of 293. A. japonicus URM5620 has a potential role in the development of a bioprocess for the XY production using low-cost media. In addition, the present study proved it is feasible to extract xylanase from SSF by adopting the one step ATPS consisting of PEG/citrate.

Keywords: Aqueous two phase systems; Aspergillus japonicus; Production; Solid-state fermentation; Xylanase.