Covalent immobilization of microbial naringinase using novel thermally stable biopolymer for hydrolysis of naringin

Ghada E A Awad; Abeer A Abd El Aty; Abeer N Shehata; Mohamed E Hassan; Magdy M Elnashar

doi:10.1007/s13205-015-0338-x

Covalent immobilization of microbial naringinase using novel thermally stable biopolymer for hydrolysis of naringin

3 Biotech. 2016 Jun;6(1):14. doi: 10.1007/s13205-015-0338-x. Epub 2016 Jan 6.

Authors

Ghada E A Awad¹, Abeer A Abd El Aty², Abeer N Shehata³, Mohamed E Hassan^{1

4}, Magdy M Elnashar^{5

6

4}

Affiliations

¹ Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt.
² Chemistry of Natural and Microbial Products Department, National Research Centre, Dokki, Giza, Egypt. aabass44@yahoo.com.
³ Biochemistry Department, National Research Centre, Dokki, Giza, Egypt. futtur@yahoo.com.
⁴ Encapsulation and Nanobiotechnology Group, Center of Excellence, National Research Centre, Dokki, Giza, Egypt.
⁵ Biomedical Sciences Department, Curtin University, Perth, Australia.
⁶ Polymers Department, National Research Centre, Dokki, Giza, Egypt.

Abstract

Naringinase induced from the fermented broth of marine-derived fungus Aspergillus niger was immobilized into grafted gel beads, to obtain biocatalytically active beads. The support for enzyme immobilization was characterized by ART-FTIR and TGA techniques. TGA revealed a significant improvement in the grafted gel's thermal stability from 200 to 300 °C. Optimization of the enzyme loading capacity increased gradually by 28-fold from 32 U/g gel to 899 U/g gel beads, retaining 99 % of the enzyme immobilization efficiency and 88 % of the immobilization yield. The immobilization process highly improved the enzyme's thermal stability from 50 to 70 °C, which is favored in food industries, and reusability test retained 100 % of the immobilized enzyme activity after 20 cycles. These results are very useful on the marketing and industrial levels.

Keywords: ART-FTIR (total reflectance Fourier transform infrared); Aspergillus niger; Immobilization; Naringinase; TGA (thermal gravimetric analysis) and biopolymer (grafted alginate gel beads).