Nonspecific protein adsorption on cationically modified Lyocell fibers monitored by zeta potential measurements

Claudia Payerl; Matej Bračič; Armin Zankel; Wolfgang J Fischer; Manuel Kaschowitz; Eleonore Fröhlich; Rupert Kargl; Franz Stelzer; Stefan Spirk

doi:10.1016/j.carbpol.2017.01.088

Nonspecific protein adsorption on cationically modified Lyocell fibers monitored by zeta potential measurements

Carbohydr Polym. 2017 May 15:164:49-56. doi: 10.1016/j.carbpol.2017.01.088. Epub 2017 Jan 25.

Authors

Claudia Payerl¹, Matej Bračič², Armin Zankel³, Wolfgang J Fischer⁴, Manuel Kaschowitz⁵, Eleonore Fröhlich⁶, Rupert Kargl⁷, Franz Stelzer⁵, Stefan Spirk⁸

Affiliations

¹ Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria; CD-Laboratory for Fiber Swelling and Paper Performance, Inffeldgasse 23(A), 8010 Graz, Austria.
² Laboratory for Characterization and Processing of Polymers (LCPP), University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
³ Graz Centre of Electron Microscopy, and Institute of Electron Microscopy, Graz University of Technology, Steyrergasse 17, 8010 Graz, Austria.
⁴ CD-Laboratory for Fiber Swelling and Paper Performance, Inffeldgasse 23(A), 8010 Graz, Austria; Institute for Paper, Pulp and Fiber Technology, Inffeldgasse 23, 8010 Graz, Austria.
⁵ Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria.
⁶ Center for Medical Research, Medical University of Graz, Stiftingtalstraße 24, Graz, Austria.
⁷ Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria; Laboratory for Characterization and Processing of Polymers (LCPP), University of Maribor, Smetanova 17, 2000 Maribor, Slovenia.
⁸ Institute for Chemistry and Technology of Materials, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria; CD-Laboratory for Fiber Swelling and Paper Performance, Inffeldgasse 23(A), 8010 Graz, Austria; Laboratory for Characterization and Processing of Polymers (LCPP), University of Maribor, Smetanova 17, 2000 Maribor, Slovenia; Institute for Paper, Pulp and Fiber Technology, Inffeldgasse 23, 8010 Graz, Austria. Electronic address: stefan.spirk@tugraz.at.

PMID: 28325343
DOI: 10.1016/j.carbpol.2017.01.088

Abstract

Nonspecific protein deposition on Lyocell fibers via a cationization step was explored by adsorption of two different N,N,N-trimethyl chitosan chlorides (TMCs). Both, the cationization and the subsequent protein deposition steps were performed and monitored in situ by evaluating the zeta potential using the streaming potential method. Both employed TMCs (degree of substitution with N⁺Me₃Cl groups: 0.27 and 0.64) irreversibly adsorb on the fibers as proven by charge reversal (-12 to +7mV for both derivatives) after the final rinsing step. Onto these cationized fibers, BSA was deposited at different pH values (4, 5, and 7). Charge titrations revealed that close to the isoelectric point of BSA (4.7), BSA deposition was particularly favored, while at lower pH values (pH 4), hardly any adsorption took place due to electrostatic repulsion of the cationic fibers and the positively charged BSA. This work sets the foundation for further investigations to use zeta potential measurements for protein adsorption studies on fibrous materials.

Keywords: Chitosan; Lyocell fibers; Protein adsorption; Tenacity; Zeta potential.

MeSH terms

Adsorption
Chitosan / chemistry*
Hydrogen-Ion Concentration
Proteins / chemistry*
Serum Albumin, Bovine / chemistry
Static Electricity
Surface Properties

Substances

N-trimethyl chitosan chloride
Proteins
Serum Albumin, Bovine
Chitosan