Imaging of Brain Slices with a Genetically Encoded Voltage Indicator

Peter Quicke; Samuel J Barnes; Thomas Knöpfel

doi:10.1007/978-1-4939-6810-7_5

Imaging of Brain Slices with a Genetically Encoded Voltage Indicator

Methods Mol Biol. 2017:1563:73-84. doi: 10.1007/978-1-4939-6810-7_5.

Authors

Peter Quicke^{1

2

3}, Samuel J Barnes³, Thomas Knöpfel^{4

5}

Affiliations

¹ Department of Bioengineering, Imperial College London, South Kensington, London, SW7 2AZ, UK.
² Centre for Neurotechnology, Imperial College London, South Kensington, London, SW7 2AZ, UK.
³ Division of Brain Sciences, Imperial College London, Hammersmith Campus, London, W12 0NN, UK.
⁴ Centre for Neurotechnology, Imperial College London, South Kensington, London, SW7 2AZ, UK. tknopfel@knopfel-lab.net.
⁵ Division of Brain Sciences, Imperial College London, Hammersmith Campus, London, W12 0NN, UK. tknopfel@knopfel-lab.net.

Abstract

Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.

Keywords: Brain slices; Fluorescence imaging; Fluorescence microscopy; Voltage imaging; Voltage indicators; Voltage sensitive fluorescent proteins.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Brain / metabolism*
Luminescent Proteins / genetics
Luminescent Proteins / metabolism*
Microscopy, Fluorescence / instrumentation
Microscopy, Fluorescence / methods*
Molecular Imaging / methods*
Voltage-Sensitive Dye Imaging / methods*

Substances

Luminescent Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding