Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)

Isabelle Ourliac-Garnier; Arturo Londoño-Vallejo

doi:10.1007/978-1-4939-6892-3_4

Telomere Strand-Specific Length Analysis by Fluorescent In Situ Hybridization (Q-CO-FISH)

Methods Mol Biol. 2017:1587:41-54. doi: 10.1007/978-1-4939-6892-3_4.

Authors

Isabelle Ourliac-Garnier^{1

2}, Arturo Londoño-Vallejo^{1

2}

Affiliations

¹ Telomeres & Cancer laboratory, CNRS-UMR3244, Institut Curie, 26 rue d'Ulm, 75248, Paris, France.
² UPMC Univ. Paris 06, F-75005, Paris, France.

PMID: 28324496
DOI: 10.1007/978-1-4939-6892-3_4

Abstract

The implementation of quantitative approaches in telomere chromosome-oriented FISH (telomeric CO-FISH) allows the assessment of the relative efficiency of lagging versus leading strand telomere replication and thus provides information on the implicated mechanisms. Here we describe a simple method for telomere strand-specific analyses and discuss its potential applications.

Keywords: CO-FISH; FISH; LNA; Lagging; Leading; PNA; Q-CO-FISH; Replication; Telomere.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosomes / genetics
Humans
In Situ Hybridization, Fluorescence / methods
Telomere / genetics*