Complete genome sequence analysis of a novel Staphylococcus phage StAP1 and proposal of a new species in the genus Silviavirus

Amal Senevirathne; Kuntal Ghosh; Eunjung Roh; Kwang-Pyo Kim

doi:10.1007/s00705-017-3316-2

Complete genome sequence analysis of a novel Staphylococcus phage StAP1 and proposal of a new species in the genus Silviavirus

Arch Virol. 2017 Jul;162(7):2145-2148. doi: 10.1007/s00705-017-3316-2. Epub 2017 Mar 21.

Authors

Amal Senevirathne¹, Kuntal Ghosh¹, Eunjung Roh², Kwang-Pyo Kim³

Affiliations

¹ Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Republic of Korea.
² Microbial Safety Team, National Institute of Agricultural Sciences, Rural Development Administration, Wanju-gun, 55365, Republic of Korea.
³ Department of Food Science and Technology, College of Agriculture and Life Sciences, Chonbuk National University, Jeonju, Jeollabuk-do, 561-756, Republic of Korea. kpkim@jbnu.ac.kr.

PMID: 28324178
DOI: 10.1007/s00705-017-3316-2

Abstract

Bacteriophage StAP1 was isolated from a soil sample infecting Staphylococcus aureus and S. xylosus. Its genome was found to be 135,502 base pairs (bp) long with 30.00 mol% G+C content and 192 open reading frames. While no tRNA encoding genes were identified, 7 mobile elements were found to interrupt five StAP1 open reading frames. Comparative genomic and proteomic analysis consistently supports the establishment of a new species in the genus Silviavirus.

MeSH terms

Base Composition
Open Reading Frames
Phylogeny*
Proteomics
Sequence Analysis, DNA
Soil Microbiology
Staphylococcus Phages / classification*
Staphylococcus Phages / genetics
Staphylococcus Phages / ultrastructure
Staphylococcus aureus / virology*
Viral Proteins / genetics

Substances

Viral Proteins