Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus

Gene. 1987;60(2-3):183-9. doi: 10.1016/0378-1119(87)90226-5.

Abstract

A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / microbiology
  • Cell Line
  • Cloning, Molecular
  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Genes, Viral*
  • Hepatitis B virus / genetics*
  • Humans
  • Liver Neoplasms
  • Plasmids
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics*
  • Viral Proteins / immunology

Substances

  • Viral Proteins