Abstract
A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carcinoma, Hepatocellular / metabolism
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Carcinoma, Hepatocellular / microbiology
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Cell Line
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Cloning, Molecular
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Fluorescent Antibody Technique
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Gene Expression Regulation
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Genes, Viral*
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Hepatitis B virus / genetics*
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Humans
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Liver Neoplasms
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Plasmids
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Viral Proteins / biosynthesis
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Viral Proteins / genetics*
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Viral Proteins / immunology