Abstract
A variety of fluorescent probes are proposed to monitor the intracellular copper content. So far, none of the probes have been evaluated for their potential to inhibit copper-associated intracellular oxidative stress. Herein, we studied the ability of a fluorescent copper probe, OBEP-CS1, to inhibit intracellular oxidative stress associated with an amyloid β (Aβ) peptide-copper complex. The data showed that OBEP-CS1 completely inhibits the copper-catalyzed oxidation as well as decarboxylation/deamination of Aβ1-16. Moreover, the cell imaging experiments confirmed that OBEP-CS1 can inhibit Aβ-CuII-catalyzed reactive oxygen species production in SH-SY5Y cells. We also demonstrated that Aβ1-16 peptide can bind intracellular copper and thereby exert oxidative stress.
MeSH terms
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Amyloid beta-Peptides / chemistry
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Amyloid beta-Peptides / metabolism*
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Catalysis
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Cell Line, Tumor
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Chelating Agents / chemistry
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Chelating Agents / pharmacology*
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Coordination Complexes / chemistry
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Coordination Complexes / metabolism
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Copper / chemistry
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Copper / metabolism*
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Fluorescent Dyes / chemistry
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Fluorescent Dyes / pharmacology*
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Humans
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Oxidation-Reduction
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Oxidative Stress / drug effects
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Peptide Fragments / chemistry
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Peptide Fragments / metabolism*
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Reactive Oxygen Species / antagonists & inhibitors*
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Reactive Oxygen Species / chemistry
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Reactive Oxygen Species / metabolism
Substances
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Amyloid beta-Peptides
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Chelating Agents
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Coordination Complexes
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Fluorescent Dyes
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Peptide Fragments
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Reactive Oxygen Species
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amyloid beta-protein (1-16)
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amyloid beta-protein (1-42)
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Copper