MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process

Ying Dong; Xintian Jin; Zhiqiang Sun; Yueming Zhao; Xianjing Song

doi:10.14348/molcells.2017.2291

MiR-186 Inhibited Migration of NSCLC via Targeting cdc42 and Effecting EMT Process

Mol Cells. 2017 Mar;40(3):195-201. doi: 10.14348/molcells.2017.2291. Epub 2017 Mar 20.

Authors

Ying Dong¹, Xintian Jin², Zhiqiang Sun³, Yueming Zhao⁴, Xianjing Song⁵

Affiliations

¹ Department of Radiotherapy, The Tumor Hospital of Jilin Province, Changchun 130021, China.
² Department of Thoracic, The Tumor Hospital of Jilin Province, Changchun 130021, China.
³ Department of Invasive Technology, The Tumor Hospital of Jilin Province, Changchun 130021, China.
⁴ Department of Oncology, The Tumor Hospital of Jilin Province, Changchun 130021, China.
⁵ Department of Cardiology, The Second Hospital of Jilin University, Changchun 130041, China.

Abstract

In this study, qRT-PCR was employed to identify that miR-186 expression level in NSCLC tissues are highly associated with lymph node metastasis. In addition, through the application of western blotting, luciferase assay and qRT-PCR, it was found that miR-186 targeted 3'UTR of cdc42 mRNA and down-regulated cdc42 protein level in a post-transcriptional manner. Transwell assay indicated that cdc42 partially reversed the effect of miR-186 mimics. Besides, miR-186 was proved to regulate EMT by influencing biomarkers of this process and cell adhesion ability. Thus, miR-186 is a potential target for NSCLC therapy. miR-186 is proposed to be one of tumor-suppressors and may serve as a therapeutic target in NSCLC treatment.

Keywords: EMT; MiR-186; NSCLC; cdc42; migration.

MeSH terms

3' Untranslated Regions
A549 Cells
Carcinoma, Non-Small-Cell Lung / genetics*
Cell Line, Tumor
Cell Movement
Cell Proliferation
Epithelial-Mesenchymal Transition
Gene Expression Regulation, Neoplastic
Humans
Lung Neoplasms / genetics*
MicroRNAs / genetics*

Substances

3' Untranslated Regions
MIRN186 microRNA, human
MicroRNAs