Few-Layer Graphdiyne Nanosheets Applied for Multiplexed Real-Time DNA Detection

Nargish Parvin; Quan Jin; Yanze Wei; Ranbo Yu; Bing Zheng; Ling Huang; Ying Zhang; Lianhui Wang; Hua Zhang; Mingyuan Gao; Huijun Zhao; Wenping Hu; Yuliang Li; Dan Wang

doi:10.1002/adma.201606755

Few-Layer Graphdiyne Nanosheets Applied for Multiplexed Real-Time DNA Detection

Adv Mater. 2017 May;29(18). doi: 10.1002/adma.201606755. Epub 2017 Mar 10.

Authors

Nargish Parvin¹, Quan Jin¹, Yanze Wei², Ranbo Yu², Bing Zheng³, Ling Huang³, Ying Zhang⁴, Lianhui Wang⁴, Hua Zhang⁵, Mingyuan Gao⁶, Huijun Zhao⁷, Wenping Hu⁸, Yuliang Li⁶, Dan Wang^{1

7}

Affiliations

¹ State Key Laboratory of Biochemical Engineering, CAS Center for Excellence in Nanoscience, Institute of Process Engineering, Chinese Academy of Sciences, No. 1 Beiertiao, Zhongguancun, Beijing, 100190, P. R. China.
² Department of Physical Chemistry, School of Metallurgical and Ecological Engineering, University of Science and Technology Beijing, No. 30, Xueyuan Road, Haidian District, Beijing, 100083, P. R. China.
³ Institute of Advanced Materials, Nanjing Tech University, Chemical Engineering Block, 5 Xinmofan Road, Nanjing, 210009, China.
⁴ School of Materials Science and Engineering, Nanjing University of Posts and Telecommunications, No. 9 Wenyuan Road, Nanjing, 210046, P. R. China.
⁵ Center for Programmable Materials, School of Materials Science and Engineering, Nanyang Technological University, No. 50 Nanyang Avenue, Singapore, 639798, Singapore.
⁶ Institute of Chemistry, Chinese Academy of Sciences, Zhongguancun North First Street 2, 100190, Beijing, P. R. China.
⁷ Centre for Clean Environment and Energy, Gold Coast Campus, Griffith University, Queensland, 4222, Australia.
⁸ Tianjin Key Laboratory of Molecular Optoelectronic Sciences, Department of Chemistry, School of Science and Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin University, 92 Weijin Road, Nankai District, Tianjin, 300072, China.

PMID: 28295711
DOI: 10.1002/adma.201606755

Abstract

Despite recent progress in 2D nanomaterials-based biosensing, it remains challenging to achieve sensitive and high selective detection. This study develops few-layer graphdiyne (GD) nanosheets (NSs) that are used as novel sensing platforms for a variety of fluorophores real-time detection of DNA with low background and high signal-to-noise ratio, which show a distinguished fluorescence quenching ability and different affinities toward single-stranded DNA and double-stranded DNA. Importantly, for the first time, a few-layer GD NSs-based multiplexed DNA sensor is developed.

Keywords: biosensing; fluorescence quenching; graphdiyne nanosheets; real-time detection.

MeSH terms

Biosensing Techniques
DNA
DNA, Single-Stranded
Nanostructures*
Spectrometry, Fluorescence

Substances

DNA, Single-Stranded
DNA