Silencing of hTERT blocks growth and migration of anaplastic thyroid cancer cells

Mol Cell Endocrinol. 2017 Jun 15:448:34-40. doi: 10.1016/j.mce.2017.03.007. Epub 2017 Mar 10.

Abstract

Mutations in the hTERT promoter responsible for constitutive telomerase activity are the most frequent genetic alteration detected in anaplastic thyroid cancer (ATC), and proposed as diagnostic and prognostic biomarker in these tumours. In this study we analyzed hTERT expression in a series of human ATCs and investigated the effects of small-interfering RNA-mediated silencing of hTERT on viability and migration and invasive properties of three human ATC cell lines. Expression of hTERT mRNA resulted increased in 8/10 ATCs compared to normal thyroid tissues. Silencing of hTERT in CAL-62, 8505C and SW1736 cells did not modify telomere length but determined a significant decrease (about 50%) of cell proliferation in all cell lines and a great reduction (about 50%) of migration and invasion capacity. These finding demonstrate that hTERT may be considered as a molecular target for ATC treatment.

Keywords: Growth inhibition; Invasion; Migration; Telomerase; Thyroid cancer; siRNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Cell Cycle
  • Cell Movement / genetics*
  • Cell Proliferation / genetics
  • Cell Survival
  • Female
  • Gene Expression Regulation, Neoplastic
  • Gene Silencing*
  • Humans
  • Male
  • Middle Aged
  • Neoplasm Invasiveness
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA, Small Interfering / metabolism
  • Telomerase / genetics*
  • Telomerase / metabolism
  • Telomere / metabolism
  • Thyroid Carcinoma, Anaplastic / enzymology*
  • Thyroid Carcinoma, Anaplastic / genetics
  • Thyroid Carcinoma, Anaplastic / pathology*

Substances

  • RNA, Messenger
  • RNA, Small Interfering
  • Telomerase