Data representing two separate LC-MS methods for detection and quantification of water-soluble and fat-soluble vitamins in tears and blood serum

Maryam Khaksari; Lynn R Mazzoleni; Chunhai Ruan; Robert T Kennedy; Adrienne R Minerick

doi:10.1016/j.dib.2017.02.033

Data representing two separate LC-MS methods for detection and quantification of water-soluble and fat-soluble vitamins in tears and blood serum

Data Brief. 2017 Feb 16:11:316-330. doi: 10.1016/j.dib.2017.02.033. eCollection 2017 Apr.

Authors

Maryam Khaksari¹, Lynn R Mazzoleni², Chunhai Ruan³, Robert T Kennedy⁴, Adrienne R Minerick¹

Affiliations

¹ Department of Chemical Engineering, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA.
² Department of Chemistry, Michigan Technological University, 1400 Townsend Drive, Houghton, MI 49931, USA.
³ Metabolomics Core, BRCF, University of Michigan, 500 South State Street, Ann Arbor, MI 48109, USA.
⁴ Department of Chemistry, University of Michigan, 500 South State Street, Ann Arbor, MI, 48109, USA.

Abstract

Two separate liquid chromatography (LC)-mass spectrometry (MS) methods were developed for determination and quantification of water-soluble and fat-soluble vitamins in human tear and blood serum samples. The water-soluble vitamin method was originally developed to detect vitamins B₁, B₂, B₃ (nicotinamide), B₅, B₆ (pyridoxine), B₇, B₉ and B₁₂ while the fat-soluble vitamin method detected vitamins A, D₃, 25(OH)D_3, E and K₁. These methods were then validated with tear and blood serum samples. In this data in brief article, we provide details on the two LC-MS methods development, methods sensitivity, as well as precision and accuracy for determination of vitamins in human tears and blood serum. These methods were then used to determine the vitamin concentrations in infant and parent samples under a clinical study which were reported in "Determination of Water-Soluble and Fat-Soluble Vitamins in Tears and Blood Serum of Infants and Parents by Liquid Chromatography/Mass Spectrometry DOI:10.1016/j.exer.2016.12.007 [1]". This article provides more details on comparison of vitamin concentrations in the samples with the ranges reported in the literature along with the medically accepted normal ranges. The details on concentrations below the limits of detection (LOD) and limits of quantification (LOQ) are also discussed. Vitamin concentrations were also compared and cross-correlated with clinical data and nutritional information. Significant differences and strongly correlated data were reported in [1]. This article provides comprehensive details on the data with slight differences or slight correlations.

Keywords: Blood serum; Fat-soluble vitamin; Infant; LC-MS method; Parent; Tears; Water-soluble vitamin.